Bile acids: analysis in biological fluids and tissues
نویسندگان
چکیده
منابع مشابه
Spectrofluorimetric determination of individual bile acids in biological fluids: duodenal content and bile.
A micromethod is described for the separation and quantitative determination of individual bile acids in duodenal content and bile. After a mild saponification procedure for splitting the taurine and glycine conjugates, the products are separated by thin-layer chromatography on silicagel layer with the solvent system //0-octane-ethyl acetate-acetic acid (60 ml + 35 ml + 5 ml) and determined by ...
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A method is described for the separation and determination of individual bile acids in serum and in intestinal contents, employing thin-layer chromatography and fluorimetry. The mean recovery from intestinal juice was 94% and from serum 82%. Normal values for the individual bile acids of serum and examples of clinical applications of the method are given.
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This review attempts to provide a concise and critical summary of modern methods for the analysis of bile acids and their conjugates in human biological fluids. Most emphasis is given to more up-to-date procedures that have been applied to the study of human disease and attention is drawn to previous reviews in areas that have not been covered here. An increasing awareness of the possibility th...
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In commonly used procedures for colorimetric assay of orotic acid in biological materials, certain substances interfere, positively or negatively. Some that interfere negatively are ornithine, cysteine, citrulline, tyrosine, proline, hydroxyproline, and polyamines. Protein and interfering agents are easily removed by first isolating the total organic acid fraction. The orotic acid in this fract...
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A simple, sensitive, and specific liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) method for the determination of bile acids in human bile has been developed. The bile acids were extracted with a C(18) (octadecyl) reversed-phase column and identified and quantified by simultaneous monitoring of their parent and daughter ions, using the multiple reaction monitoring mode. I...
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ژورنال
عنوان ژورنال: The Journal of Lipid Research
سال: 2009
ISSN: 0022-2275
DOI: 10.1194/jlr.r001941-jlr200